Search results for " Klebsiella pneumoniae"

showing 10 items of 18 documents

Co-existence of virulence factors and antibiotic resistance in new Klebsiella pneumoniae clones emerging in south of Italy

2018

Abstract Background Endemic presence of Klebsiella pneumoniae resistant to carbapenem in Italy has been due principally to the clonal expansion of CC258 isolates; however, recent studies suggest an ongoing epidemiological change in this geographical area. Methods 50 K. pneumoniae strains, 25 carbapenem-resistant (CR-Kp) and 25 susceptible (CS-Kp), collected from march 2014 to march 2016 at the Laboratory of Bacteriology of the Paolo Giaccone Polyclinic University hospital of Palermo, Italy, were characterized for antibiotic susceptibility and fully sequenced by next generation sequencing (NGS) for the in silico analysis of resistome, virulome, multi-locus sequence typing (MLST) and core sin…

0301 basic medicineAdultKlebsiellaGenotypeKlebsiella pneumoniae030106 microbiologyVirulenceYersiniabactinPolymorphism Single Nucleotidebeta-Lactamaseslcsh:Infectious and parasitic diseasesMicrobiology03 medical and health scienceschemistry.chemical_compoundBacterial ProteinsGenotypeDrug Resistance BacterialHumanslcsh:RC109-216TypingSicilyPhylogenyAgedAged 80 and overbiologyVirulence factorsMiddle Agedbiology.organism_classificationAnti-Bacterial AgentsBacterial Typing TechniquesKlebsiella InfectionsKlebsiella pneumoniae030104 developmental biologyInfectious DiseaseschemistryCarbapenemsCarbapenem-resistant Klebsiella pneumoniaeMultilocus sequence typingAerobactinMultilocus Sequence TypingResearch Article
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Efficacy of a coordinated strategy for containment of multidrug-resistant Gram-negative bacteria carriage in a Neonatal Intensive Care Unit in the co…

2021

AbstractBackgroundAntimicrobial resistance in neonatal intensive care unit (NICU) patients is a threat, due to the frequent use of antimicrobial treatment and invasive devices in fragile babies. Since 2014 an active surveillance program of multidrug-resistant Gram-negative bacteria (MDR-GNB) carriage has been in place in the five NICUs of Palermo, Italy. In 2017 an increase in the prevalence of MDR-GNB, and in particular of extended-spectrum β-lactamases-producingKlebsiella pneumoniae(ESBL-KP), was observed in “Civico” hospital NICU.AimTo assess the impact of a coordinated intervention strategy in achieving long-lasting reduction of MDR-GNB prevalence in the NICU.MethodsRectal swabs were ob…

0301 basic medicineMicrobiology (medical)Malemedicine.medical_specialtyNeonatal intensive care unit030106 microbiologyExtended spectrum β lactamases producing klebsiella pneumoniaeContext (language use)Drug resistanceMicrobial Sensitivity TestsAntimicrobial resistancelcsh:Infectious and parasitic diseases03 medical and health sciences0302 clinical medicineAntibiotic resistanceMedical microbiologyNeonatal intensive care unitInternal medicineDrug Resistance Multiple BacterialIntensive Care Units NeonatalGram-Negative BacteriamedicineHumansPharmacology (medical)lcsh:RC109-216030212 general & internal medicineNeonatal intensive care unit.Cross InfectionInfection ControlActive surveillance programbusiness.industryResearchPublic Health Environmental and Occupational HealthInfant NewbornAntimicrobialMulti-drug resistant gram-negative bacteriaElectrophoresis Gel Pulsed-FieldInfectious DiseasesCarriageItalyIntervention strategyCarrier StateFemaleSample collectionbusinessGram-Negative Bacterial Infections
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Molecular epidemiology of KPC-producing Klebsiella pneumoniae from invasive infections in Italy: Increasing diversity with predominance of the ST512 …

2016

Objectives The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanita surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP). Methods A total of 17 laboratories distributed across Italy collected all consecutive non-replicate CR-KP isolated from invasive infections during two different s…

0301 basic medicineMicrobiology (medical)gelKlebsiella pneumoniaegenotype030106 microbiologyPopulationcross-sectional studiespulsed-fieldmultilocus sequence typingcross-sectional studies; electrophoresis gel pulsed-field; humans; italy; klebsiella infections; klebsiella pneumoniae; molecular epidemiology; multilocus sequence typing; serogroup; beta-lactamases; genetic variation; genotypeSettore BIO/19 - Microbiologia GeneraleCross-Sectional Studies; Electrophoresis Gel Pulsed-Field; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Serogroup; beta-Lactamases; Genetic Variation; Genotype; Pharmacology; Microbiology (medical); Pharmacology (medical); Infectious Diseasesmolecular epidemiologylaw.inventionMicrobiologybeta-Lactamasebeta-lactamases03 medical and health scienceslawGenotypeitalyPulsed-field gel electrophoresisPharmacology (medical)TypingeducationhumansPolymerase chain reactionPharmacologyCross-Sectional Studieeducation.field_of_studybiologyMolecular epidemiologybiochemical phenomena metabolism and nutritionbiology.organism_classificationSettore MED/07 - Microbiologia e Microbiologia Clinicabacterial infections and mycosesVirologyElectrophoresis Gel Pulsed-Fieldklebsiella pneumoniaeCross-Sectional Studies; Electrophoresis Gel Pulsed-Field; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Serogroup; beta-Lactamases; Genetic Variation; Genotype; Pharmacology; Pharmacology (medical); Infectious DiseasesInfectious Diseasesklebsiella infectionselectrophoresisgenetic variationMultilocus sequence typingserogroupHumanKlebsiella Infection
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Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone

2017

ABSTRACT : The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and t…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinicasiderophoreantibiotic resistancelong term survivalsequence analysisKlebsiella pneumoniaepolymerase chain reactionResponses to Human InterventionsDrug ResistanceGene TransferClone (cell biology)ST259bacterial proteinvirulence factorYersiniabactinGenomechemistry.chemical_compoundMicrobialPlasmidAntibioticsbacterial genomepathogenicitygenetics610 Medicine & healthgenome analysisCross InfectionMolecular EpidemiologyGenomeVirulencebiologydrug effectyersiniabactinBacterialDrug Resistance MicrobialGeneral MedicineKlebsiella infectionglycogen synthesisKlebsiella pneumoniaeEnglandItalyST307horizontal gene transferProteínas BacterianasResearch ArticleGene Transfer HorizontalVirulence FactorsSequence analysiscapsule030106 microbiologyVirulence610 Medicine & healthpulsed field gel electrophoresisColombiaCarbapenemase; siderophore; yersiniabactin; bacterial protein; beta lactamase; virulence factor antibiotic resistance; Article; bacterial strain; bacterial virulence; bacterium isolate; fimbria; genome analysis; glycogen synthesis; Klebsiella pneumoniae; long term survival; microbial diversity; nonhuman; plasmid; polymerase chain reaction; pulsed field gel electrophoresis; sequence analysis; whole genome sequencing; antibiotic resistance; bacterial genome; carbapenem-resistant Enterobacteriaceae; Colombia; cross infection; drug effect; England; genetic variation; genetics; horizontal gene transfer; human; Italy; Klebsiella infection; microbiology; molecular epidemiology; multilocus sequence typing; pathogenicity; virulence Bacterial Proteins; beta-Lactamases; Carbapenem-Resistant Enterobacteriaceae; Colombia; Cross Infection; Drug Resistance Microbial; England; Gene Transfer Horizontal; Genetic Variation; Genome Bacterial; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Virulence; Virulence Factors; Whole Genome SequencingArticlebeta-Lactamasesbeta lactamaseHorizontalMicrobiologyCarbapenemase03 medical and health sciencesAntibiotic resistanceBacterial ProteinsplasmidHumanshumanInfecciones por KlebsiellafimbrianonhumanWhole Genome Sequencingbacterial virulencebacterium isolatemicrobiologyGenetic Variationbacterial strainbiology.organism_classificationKlebsiella InfectionsEnterobacteriaceae Resistentes a los CarbapenémicosKPCCarbapenem-Resistant Enterobacteriaceae030104 developmental biologychemistrymicrobial diversityEpidemiología MolecularGenome BacterialWGSMultilocus Sequence Typing
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Hypervirulent Klebsiella pneumoniae Endogenous Endophthalmitis—A Global Emerging Disease

2021

The review aims to document the new emerging hypervirulent Klebsiella pneumoniae (Kp) endogenous endophthalmitis (EKE) in terms of incidence, microbiological characterization of the pathogenic agent, associated risk factors, management, and outcomes. Hypervirulent (hv) strains of KP (hvKp) induce invasive liver abscesses (LA) with specific clinical features. Up to 80–90% of cases have hepatic liver abscess as a primary focus of infection, followed by renal or lung hvKp infections. However, the incidence of EKE in patients with KPLA varied between 3.4% (19) and 12.6% (13), with a total of 95 cases of endophthalmitis in 1455 cases of KPLA (6.5%). Severe visual loss was encountered in 75% of c…

0301 basic medicinemedicine.medical_specialtymedicine.drug_classKlebsiella pneumoniaeScience030106 microbiologyCephalosporinDiseaseReviewGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesTherapeutic approach0302 clinical medicineEndophthalmitisAntibiotic resistanceInternal medicinemedicineEcology Evolution Behavior and Systematicshypervirulent Klebsiella pneumoniaepercutaneous drainagebiologybusiness.industryIncidence (epidemiology)intravitreal antibiotherapyQPaleontologymedicine.diseasebiology.organism_classificationhypervirulent <i>Klebsiella pneumoniae</i>endophthalmitisSpace and Planetary Science030221 ophthalmology & optometrybusinessinvasive liver abscess syndromeLiver abscessearly diagnosisLife
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Co-colonization with carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii in intensive care unit patients

2013

Objectives: This investigation was conducted to study co-colonization by carbapenem-resistant Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) and Acinetobacter baumannii (CRAB) in intensive care unit (ICU) patients in Palermo, Sicily, a geographic area where both organisms are endemic in the healthcare setting. Risk factors at admission and during ICU stay and outcomes were also evaluated. Methods: All patients colonized by KPC-Kp, or CRAB, or both in 2 ICUs of a large general hospital during the period October 2011 – March 2012 were enrolled. Demographics and clinical data were collected. Resistance determinants and clonality of the 2 organisms were characterized by mo…

Acinetobacter baumanniiAdultMaleMicrobiology (medical)medicine.medical_specialtyPediatricsYounger ageGenotypeCarbapenem resistant Klebsiella pneumoniaeKlebsiella pneumoniaeCritical IllnessSettore MED/42 - Igiene Generale E ApplicataHospitals Generalbeta-Lactamaseslaw.inventionBacterial ProteinsRisk FactorslawInternal medicineDrug Resistance BacterialmedicineHumansGeneral hospitalSicilyAgedAged 80 and overGeneral Immunology and MicrobiologybiologyCoinfectionbusiness.industryMajor traumaGeneral MedicineMiddle Agedbiology.organism_classificationmedicine.diseaseIntensive care unitCarbapenem resistance co-colonization intensive care unit risk factorsKlebsiella InfectionsAcinetobacter baumanniiMolecular TypingIntensive Care UnitsKlebsiella pneumoniaeInfectious DiseasesFemaleCo colonizationbusinessAcinetobacter InfectionsScandinavian Journal of Infectious Diseases
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OXA-163-Producing Klebsiella pneumoniae in Cairo, Egypt, in 2009 and 2010

2012

ABSTRACT Two genetically unrelated OXA-163-carrying Klebsiella pneumoniae strains were identified from two infection cases in June 2009 and May 2010 in Cairo, Egypt. OXA-163-producing Enterobacteriaceae had been previously reported in Argentina only. Both patients had no history of travel abroad. The emergence of this newly recognized OXA-48-related β-lactamase able to hydrolyze cephalosporins and carbapenems is especially worrying in a geographic area where OXA-48 is endemic and effective surveillance for antibiotic resistance is largely unaffordable.

AdultDNA BacterialMaleMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaOXA-163EpidemiologyKlebsiella pneumoniaemedicine.drug_classMolecular Sequence DataCephalosporinSettore MED/42 - Igiene Generale E Applicatabeta-Lactam Resistancebeta-LactamasesTravel abroadMicrobiologyAntibiotic resistancepolycyclic compoundsmedicineHumansOXA-163; Klebsiella pneumoniaebiologyGeographic areaHydrolysisSequence Analysis DNAKlebsiella infectionsbiochemical phenomena metabolism and nutritionbiology.organism_classificationVirologyAnti-Bacterial AgentsCephalosporinsKlebsiella InfectionsKlebsiella pneumoniaeCarbapenemsbacteriaEgyptFemaleJournal of Clinical Microbiology
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An Update of the Evolving Epidemic of blaKPC Carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of Multiple Non-ST258 Clones

2015

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute…

CarbapenemKlebsiella pneumoniaelcsh:MedicineGene ExpressionDrug resistancePlasmidbeta-LactamaseDisease OutbreaksMolecular typingFluoroquinoloneDrug Resistance Multiple Bacterialpolycyclic compoundslcsh:ScienceCarbapenemMembrane ProteinDisease OutbreakMultidisciplinarybiologyMedicine (all)IncidenceHospitalsAnti-Bacterial AgentsElectrophoresis Gel Pulsed-FieldKlebsiella pneumoniaeItalyEpidemiological MonitoringHumanFluoroquinolonesPlasmidsResearch Articlemedicine.drugBacterial ProteinAminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Colistin; Drug Resistance Multiple Bacterial; Electrophoresis Gel Pulsed-Field; Epidemiological Monitoring; Fluoroquinolones; Gene Expression; Hospitals; Humans; Incidence; Italy; Klebsiella Infections; Klebsiella pneumoniae; Membrane Proteins; Multilocus Sequence Typing; Mutation; Plasmids; beta-Lactamases; Disease Outbreaks; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)beta-LactamasesMicrobiologyClone CellHospitalAntibiotic resistanceBacterial ProteinsAnti-Bacterial AgentmedicineHumansBiochemistry Genetics and Molecular Biology (all)AminoglycosideColistinlcsh:RMembrane ProteinsCarbapenemase producingbiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyClone CellsKlebsiella InfectionsAminoglycosidesAgricultural and Biological Sciences (all)CarbapenemsMutationColistinMultilocus sequence typinglcsh:QKlebsiella InfectionMultilocus Sequence TypingPLOS ONE
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Ceftazidime-avibactam use for klebsiella pneumoniae carbapenemase-producing k. pneumoniae infections: A retrospective observational multicenter study

2021

Abstract Background A growing body of observational evidence supports the value of ceftazidime-avibactam (CAZ-AVI) in managing infections caused by carbapenem-resistant Enterobacteriaceae. Methods We retrospectively analyzed observational data on use and outcomes of CAZ-AVI therapy for infections caused by Klebsiella pneumoniae carbapenemase–producing K. pneumoniae (KPC-Kp) strains. Multivariate regression analysis was used to identify variables independently associated with 30-day mortality. Results were adjusted for propensity score for receipt of CAZ-AVI combination regimens versus CAZ-AVI monotherapy. Results The cohort comprised 577 adults with bloodstream infections (n = 391) or nonba…

Microbiology (medical)Adultmedicine.medical_specialtyAzabicyclo CompoundcarbapenemasesBacterial ProteinMicrobial Sensitivity TestsNeutropeniaCeftazidimebeta-Lactamasesbeta-LactamaseCarbapenemasecarbapenemaseBacterial ProteinsRetrospective StudieLower respiratory tract infectionInternal medicineDrug CombinationAnti-Bacterial AgentmedicineHumansKPC-producing Klebsiella pneumoniaeRetrospective StudiesSeptic shockbusiness.industryCeftazidime-avibactamMicrobial Sensitivity Testceftazidime-avibactamMortality rateCarbapenemases; Ceftazidime-avibactam; KPC-producing Klebsiella pneumoniae; Adult; Anti-Bacterial Agents; Azabicyclo Compounds; Bacterial Proteins; Ceftazidime; Drug Combinations; Humans; Microbial Sensitivity Tests; Retrospective Studies; beta-Lactamases; Klebsiella Infections; Klebsiella pneumoniaeKPC-producing Klebsiella pneumoniae; carbapenemases; ceftazidime-avibactammedicine.diseaseCeftazidime/avibactamSettore MED/17KPC-producing Klebsiella pneumoniae; carbapenemases; ceftazidime-avibactam; Adult; Anti-Bacterial Agents; Azabicyclo Compounds; Bacterial Proteins; Ceftazidime; Drug Combinations; Humans; Microbial Sensitivity Tests; Retrospective Studies; beta-Lactamases; Klebsiella Infections; Klebsiella pneumoniaeAnti-Bacterial AgentsKlebsiella InfectionsDrug CombinationsKlebsiella pneumoniaeInfectious DiseasesCohortPropensity score matchingObservational studybusinessAzabicyclo Compoundsmedicine.drugHumanKlebsiella Infection
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NDM-1 and OXA-163 producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012

2013

Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the blaNDM-1 gene and the second isolate carried the blaOXA-163 gene. Both isolates co-expressed the extended-spectrum β-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of …

Microbiology (medical)Antimicrobial drug resistanceSettore MED/07 - Microbiologia E Microbiologia ClinicaOXA-163biologyGeographic areaKlebsiella pneumoniaeImmunologyNorth africaSequence typesNDM-1biology.organism_classificationMicrobiologyVirologyMicrobiologyCarbapenemaseKlebsiella pneumoniaeNDM-1; OXA-163; Carbapenemase; Klebsiella pneumoniae; Antimicrobial drug resistancePulsed-field gel electrophoresisImmunology and AllergyMultilocus sequence typingCarbapenem resistance
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